SEED BIOLOGY
UNDERSTANDING HOW THESE COMPLEX ENTITIES FUNCTION
RECENT PUBLICATIONS
2021
Yumin Zhang, Dan Li, Lynnette M. A. Dirk, A. Bruce Downie, Tianyong Zhao. “ZmAGA1 Hydrolyzes RFOs Late during the Lag Phase of Seed Germination, Shifting Sugar Metabolism toward Seed Germination Over Seed Aging Tolerance.” Journal of Agricultural and Food Chemistry. 69(39):11606-11615. doi: 10.1021/acs.jafc.1c03677

2020  
Qinghui Han, Kelu Chen, Dong Yan, Guanglong Hao, Junlong Qi, Chunmei Wang, Lynnette M.A. Dirk, A. Bruce Downie, Jianhua Gong, Jianhua Wang, Tianyong Zhao. "ZmDREB2A regulates ZmGH3.2 and ZmRAFS, shifting metabolism towards seed aging tolerance over seedling growth." The Plant Journal. 104:268-282. doi: 10.1111/tpj.14922  
 
Ran Tian, Fangfang Wang, Qiaolin Zheng, Venus M. A. G. E. Niza, A. Bruce Downie, Sharyn E. Perry. "Direct and indirect targets of the arabidopsis seed transcription factor ABSCISIC ACID INSENSITIVE3." The Plant Journal. 103:1679-1694. doi: 10.1111/tpj.14854 
 
Lynnette M.A. Dirk, Caser Ghaafar Abdel, Imran Ahmad, Izabel Costa Silva Neta, Cristiane Carvalho Pereira, Francisco Elder Carlos Bezerra Pereira, Sandra Helena Unêda-Trevisoli, Daniel Guariz Pinheiro, Allan Bruce Downie. “LATE EMBRYOGENESIS ABUNDANT PROTEIN-CLIENT PROTEIN interactions.” Plants. 9(7):814. doi: 10.3390/plants9070814  
 
Tao Li, Yumin Zhang, Ying Liu, Xudong Li, Guanglong Hao, Qinghui Han, Lynnette M.A. Dirk, A. Bruce Downie, Yong-Ling Ruan, Jianmin Wang, Guoying Wang, Tianyong Zhao. "Raffinose synthase enhances drought tolerance through raffinose synthesis or galactinol hydrolysis in maize and Arabidopsis plants." Journal of Biological Chemistry. 295(23):8064-8077. doi: 10.1074/jbc.RA120.013948 
 
Qinghui Han, Junlong Qi, Guanglong Hao, Chunxia Zhang, Chunmei Wang, Lynnette M.A. Dirk, A. Bruce Downie, Tianyong Zhao. "ZmDREB1A regulates RAFFINOSE SYNTHASE controlling raffinose accumulation and vegetative chilling stress tolerance in maize." Plant & Cell Physiology. 61(2):331-341. doi: 10.1093/pcp/pcz200 
 
2019  
Lei Gu, Tao Jiang, Chunxia Zhang, Xudong Li, Chunmei Wang, Yumin Zhang, Tao Li, Lynnette M.A. Dirk, A. Bruce Downie, Tianyong Zhao. “Maize HSFA 2 and HSBP 2 antagonistically modulate raffinose biosynthesis and heat tolerance in Arabidopsis.” The Plant Journal. 100(1):128-142. doi: 10.1111/tpj.14434 
 
Yumin Zhang, Qichao Sun, Chunxia Zhang, Guanglong Hao, Chunmei Wang, Lynnette M.A. Dirk, A. Bruce Downie, and Tianyong Zhao. "Maize VIVIPAROUS1 interacts with ABA INSENSITIVE5 to regulate GALACTINOL SYNTHASE2 expression controlling seed raffinose accumulation." Journal of Agricultural and Food Chemistry. 67(15):4214-4223. doi: 10.1021/acs.jafc.9b00322
 

2018
Lynnette M. A. Dirk, and A. Bruce Downie. 2018. "An examination of Job’s rule: Protection and repair of the proteins of the translational apparatus in seeds." Seed Science Research. 28, 168–181.
  
Lynnette M.A. Dirk, Manoj Majee, Santosh Kumar, A. Bruce Downie. 2018. "PHYTOCHROME INTERACTING FACTOR1 interactions leading to the completion or prolongation of seed germination." Plant Signaling and Behavior. 13(10):e1525999.
 
Manoj Majee, Santosh Kumar, Praveen Kumar Kathare, Shuiqin Wu, Derek Gingerich, Nihar R Nayak, Louai Salaita, Randy Dinkins, Kathleen Martin, Michael Goodin, Lynnette M.A. Dirk, Taylor D. Lloyd, Ling Zhu, Joseph Chappell, Art G. Hunt, Richard Vierstra, Enamul Huq, and A. Bruce Downie. 2018. "KELCH F-BOX Protein Positively Influences Seed Germination by Targeting PHYTOCHROME-INTERACTING FACTOR1." Proceedings of the National Academy of Science U.S.A. 115 (17) E4120-E4129. 

2017
Tao Li, Yumin Zhang, Dong Wang, Ying Liu, Lynnette M.A. Dirk, Jack Goodman, A. Bruce Downie, Jianmin Wang, Guoying Wang, Tianyong Zhao. "Regulation of Seed Vigor by Manipulation of Raffinose Family Oligosaccharides (RFOs) in Maize and Arabidopsis." Molecular Plant. 10(12):1540:1555.

Majee Manoj, Wu Shuiqin, Salaita Louai, Gingerich Derek, Dirk Lynnette M.A., Chappell Joseph, Hunt Art G., Vierstra Richard, Downie A. Bruce. "A Misannotated Locus Positively Influencing Arabidopsis Seed Germination is Deconvoluted Using Multiple Methods, Including surrogate Splicing." Plant Gene. 10: 74-85. 

Santos, Juliana F., Dirk, Lynnette M.A., Downie, A. Bruce, Sanches, Mauricio F. G. and Vieira, Roberval D. "Soluble Sugars, Storage Proteins and Reciprocal Effect of Parental Lines of Corn Hybrid Seeds." Seed Science Research. 27: 206-216. 
 
"Findings from Sao Paulo State University Broaden Understanding of Seed Science." (Reciprocal Effect of Parental Lines on the Physiological Potential and Seed Composition of Corn Hybrid Seeds). 
Agriculture Week. Sep 21, 2017, p.106. 

2016
Gu Lei, Zhang Y., Zhang M., Li Tao, Dirk Lynnette M.A., Downie B., Zhao T-Y. "ZmGOLS2, a Target of Transcription Factor ZmDREB2A, Offers Similar Protection Against Abiotic Stress as ZmDREB2A, When Either is Over-Expressed in Arabidopsis, While Presenting a Wild Type Growth Habit in Unstressed kConditions." Plant Molecular Biology. 90:157–170. 

2014
Han Q, Li T, Zhang L, Dirk Lynnette M.A, Downie B, Zhao T-Y. "Functional Analysis of the 5’ Regulatory Region of the Maize ALKALINE ALPHA-GALACTOSIDASE1 Gene." Plant Molecular Biology Reporter. 33: 1361-1370. DOI 10.1007/s11105-014-0840-z. 

Barrero J.M., Downie A.B., Xu Q, Gubler F. "A Role for Barley CRYPTOCHROME1 in Light Regulation of Grain Dormancy and Germination." The Plant Cell. 26:1094-1104.  In this issue: "Cryptochromes and Seed Dormancy: The Molecular Mechanism of Blue Light Inhibition of Grain Germination." Nancy Hofmann. The Plant Cell. 2014; 26: 846.

Kushwaha R, Schäfermeyer K.R., Downie A.B. "A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits." Journal of Visualized Experiments. 84, e50685, doi:10.3791/50685. 
 
2013
Petti C., Harman-Ware A.E., Tateno M., Kushwaha R., Shearer A., Downie A.B., Crocker M., DeBolt S. "Sorghum Mutant RG Displays Antithetic Leaf Shoot Lignin Accumulation Resulting in Improved Stem Saccharification Properties." Biotechnology for Biofuels. 6: 146. 

Gu Lei, Han Z., Zhang ., Downie B., Zhao T-Y. Functional Analysis of the 5’ Regulatory Region of the Maize GALACTINOL SYNTHASE2 Gene. Plant Science. 213: 38–45. 

Nayak N., Putnam A., Chen T., Addepalli B., Lowenson J., Jankowsky E., Perry S.E., Dinkins R., Limbach P., Clarke S.G., Downie A.B. "An ATP Dependent DEAD-box RNA-helicase Loses Activity Upon isoAsp Formation but is Restored by PROTEIN ISOASPARTYL METHYLTRANSFERASE. The Plant Cell. 25:2573-2586.